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Information × Registration Number 0224U032896, (0123U102674) , R & D reports Title Therapeutic potential and safety of three-dimensional constructs based on mesenchymal stem cells for treatment of non-healing wounds popup.stage_title Head Petrenko Oleksandr Yu., Доктор біологічних наук Registration Date 10-12-2024 Organization Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine popup.description1 The aim of the project is to investigate the proliferative, metabolic and paracrine activity of human mesenchymal stem cells in monolayer and three-dimensional conditions (gel, spheroids, capsules and scaffolds) and their reparative action on nonhealing wound model; to establish optimal conditions and storage period for ready-to-use bioconstructs with high efficiency. popup.description2 The objects of research are mesenchymal stem/stromal cells in suspension and as part of three-dimensional bioconstructions. The aim of the work was to investigate the effect of storage periods at ambient temperature of human MSCs in alginate capsules, macroporous scaffolds and spheroids on the viability, metabolic and proliferative activity of cells and the level of oxidative stress. Micro- and macroscopic, preparative, biochemical, spectrophotometric, fluorimetric, and cultivation methods were used in the work. It was shown that during the storage of human adipose tissue mesenchymal stromal cells (MSCs) in three different 3D structures (alginate capsules, macroporous scaffolds, spheroids) for 3 days at a temperature of 22°C, there is no significant loss of viability, morphological integrity, proliferative and differentiating potential in contrast to the suspension. It was found that during the formation of spheroids, as well as after encapsulation of MSCs in alginate capsules, the metabolic activity of cells decreases, which increases their resistance to further storage at ambient temperature. During cultivation in scaffolds, cells have a high level of metabolic activity, which decreases during further storage, but recovers after returning to cultivation conditions. It was found that the storage of MSCs in suspension and 3D structures for 3 days has almost no effect on the production of reactive oxygen species, but after 7 days of storage, this indicator increases rapidly only in suspension and scaffolds. Taking into account the obtained results, it was established that the use of 3D structures increases the resistance of MSCs to damaging factors of storage at ambient temperature. Optimal storage terms were chosen – creation of a microenvironment in alginate capsules, scaffolds and spheroids allows maintaining the main structural and functional properties of MSCs when stored for at least 3 days at ambient temperature. Product Description popup.authors Petrenko Oleksandr Yu. Revenko Olena B. Mazur Svitlana P. Trufanova Nataliia A. Bozhok Halyna A. Pakhomov Oleksandr V. Cherkashyna Daria V. popup.nrat_date 2024-12-10 Close