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0224U033105,
(0124U002066)
,
Development of new hybridization DNA sensors modified by nanomaterials based on surface plasmon resonance
Розробка нової технології створення модифікованої НЧЗ біосенсорної системи на основі ППР для визначення 80-mer BCR-ABL у біологічних рідинах
Kucherenko Ivan S., Кандидат біологічних наук
18-12-2024
Institute of Molecular Biology and Genetics of NAS of Ukraine
The aim of the work is to develop a new technology for using nanomaterials to create DNA biosensors based on PPR with improved analytical characteristics.
As a result of the project, the possibility of using gold nanoparticles (GNPs) to improve the sensory characteristics of DNA biosensors based on surface plasmon resonance (SPR) spectrometry was tested. The possibility of using 6-mercapto-1-hexanol and lipoic acid to modify the surface of GNPs was analyzed in order to improve their colloidal stability in chemical conditions that allow DNA hybridization. The colloidal stability of nanoparticles before and after modification in various chemical environments was studied. The possibility of using the studied GNPs in biosensor analysis was shown, and the optimal conditions for their functioning and long-term storage were determined. A new design of a hybridization DNA biosensor with modified GNPs based on SPR spectrometry was tested. Biosensor systems operating using modified GNPs were characterized by better sensitivity to DNA markers than a traditional DNA biosensor. Based on the data obtained during the study, the prospects of using modified NSCs as components of DNA hybridization systems for detecting genetic markers have been proven.
Kucherenko Daria Yu.
Mruha Daryna O.
Sobolevskyi Maksym S.
2024-12-18
Updated: 2025-12-26
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