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Information × Registration Number 0224U033526, (0124U004087) , R & D reports Title Study of genetic variability of bryophytes from the Maritime Antarctic using molecular genetic markers popup.stage_title Вивчення генетичної мiнливостi мохоподiбних з Морської Антарктики з використанням молекулярно-генетичних маркерів Head Blium Yaroslav B., Доктор біологічних наук Registration Date 30-12-2024 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description1 The aim of the work is to characterize the molecular genetic differences of natural populations of C. relaxa from the Maritime Antarctic by evaluating the polymorphism of the intron length of the β-tubulin genes and individual sections of the nuclear and chloroplast genomes popup.description2  During the project, samples of Cratoneuropsis relaxa belonging to different populations were studied. In particular, a molecular genetic analysis of 19 natural populations of C. relaxa from 8 islands of the Maritime Antarctic was carried out by assessing the length polymorphism of introns of β-tubulin genes using known and self-developed primer pairs. As a result of the analysis, a low level of molecular genetic polymorphism of C. relaxa was established. At the same time, samples from the most remote northern and southern islands (Booth and Charvez) are characterized by the presence of unique amplicons in their DNA profile, and for some samples (Black and Petermann) from the central part of the studied region, a combination of typical and unique fragments is observed. All this probably indicates the possible genetic isolation of the marginal populations of C. relaxa and the more severe conditions of existence that cause adaptive divergence, which increases genetic diversity. The lack of polymorphism in populations from the central part and the presence of populations that combine samples with typical and distinctive DNA profiles may indicate an intermediate position between the extreme populations or genetic flow between the individual groups of samples studied. The results of the sequencing of ITS1 and rps4 fragments showed that ITS1 is characterized by greater polymorphism than rps4, which turned out to be insufficiently variable to detect differences between C. relaxa populations. In general, a low number of mutations was found, which indicates the stability of populations that are subject to strong selection, which can reduce diversity by fixing certain allelic states. Product Description popup.authors Blium Rostyslav Ya. Novozhylov Dmytro O. Pirko Yaroslav V. Rabokon Anastasiia M. Sakharova Vladyslava H. popup.nrat_date 2024-12-30 Close