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Information × Registration Number 0225U000834, (0124U001934) , R & D reports Title Investigation of the regenerative capacity of human multipotent stem cells using a temporary equivalent of the dermal layer of the skin to improve the treatment of burn and wound surfaces popup.stage_title Створити експериментальну модель in vitro стовбурових клітин людини, визначити життєздатність стовбурових клітин різного походження (виживаність, проліферативну та мітотичну активність), іммобілізованих в гідрогель та без нього Head Lavrenchuk Halyna Yo., д.б.н.Talko Viktoria V., д.мед.н. Registration Date 20-01-2025 Organization State Institution "National Research Center for Radiation Medicine, Hematology and Onkology of the National Academy of Medical Sciences of Ukraine" popup.description1 To investigate the regenerative activity of human stem cells of various origins, immobilized in the dermal equivalent of the skin, in the process of healing burn and wound surfaces popup.description2  The object of the study: biotechnological wound coverings (derma equivalents) with the inclusion of human stem cells of various established lineages or their derivatives, intended for the treatment of burn disease and other wounds. The purpose of the work: to create an experimental model of human stem cells in vitro, to determine the viability of stem cells of various origins (survival, proliferative and mitotic activity), and to investigate the effectiveness of stem cell immobilization in the temporary equivalent of the dermal layer of the skin using hydrogel. Research methods: cultivation of human stem cells; light microscopy to assess the qualitative and quantitative characteristics of cells; determination of cell viability by staining with trypan blue; development and cryopreservation of biomaterials (cells of established lines and cell-free media conditioned by cells in culture (BCS); preparation and adaptation of cell carriers, hydrogels and membranes from materials of natural and artificial origin; creation of combined bioconstructions (carriers containing cells or BKS), methods of statistical data analysis.As a result of the work, new biotechnological products were created: a) using cell technology, equivalents of the dermal layer of the skin were obtained with the inclusion of human cells of the original lines or their derivatives (conditioned media containing a complex of biologically active substances synthesized by cells in culture); b) it is shown that dermal coatings based on a collagen membrane ("Bilkosin" film) and gelatin hydrogel containing immobilized stem cells or a derivative of these cells, BCS, can be the most effective bioconstructions that contribute to accelerated wound healing. This greatly simplifies and makes the technology of obtaining and using new dermis equivalents more economical. Product Description popup.authors Dmytriieva Inna R. Malyshevska Yevheniia M. Oleinikova Tetiana H. Chernyshov Andrii V. popup.nrat_date 2025-01-20 Close