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Information × Registration Number 0225U001211, (0123U101651) , R & D reports Title Development of a technology for obtaining and using platelet-rich plasma for the treatment of hemophilia patients with arthropathies popup.stage_title Розробити спосіб отримання плазми, багатої тромбоцитами із крові хворих на гемофілію та алгоритм її використання у цих пацієнтів. Head Averianov Yevhen V., Кандидат медичних наук Registration Date 27-01-2025 Organization State Institution "National Research Center for Radiation Medicine, Hematology and Onkology of the National Academy of Medical Sciences of Ukraine" popup.description1 To create a technology for obtaining platelet-rich autoplasma of blood from hemophilia patients for use in the treatment of arthropathies in this category of patients popup.description2  The purpose is to develop a method of obtaining plasma rich in platelets (Pl) from the blood of patients with hemophilia and the algorithm of its use in these patients. Research methods – clinical, biochemical, hematological, mathematical. A method of obtaining autologous platelet-rich plasma (PRP) from the blood of hemophiliacs has been developed and the algorithm of its use for the treatment of hemophilic arthropathies (HA) based on the conducted study of quantitative and functional indicators of Pl in these patients. No significant difference was found in the number of Pl and their aggregation functions in relation to similar parameters of the control group, which made it possible to use the blood of hemophiliacs for the production of autologous preparations of PRP for use as a therapeutic agent to accelerate regenerative processes in joints affected by HA. Research has been conducted by influence of different centrifugation modes (150 g, 500 g and 1000 g) and it was established that the maximum concentration of Pl is determined in plasma samples obtained at the 150 g and 500 g centrifugation modes in tubes with different types of anticoagulants. A correlation between an increase in relative centrifugal force during centrifugation and an increase in platelet aggregation indicators was revealed. When using tubes with sodium heparin, the increase in platelet aggregation indicators in samples of PRP was greater compared to samples obtained from tubes with sodium citrate and when the relative centrifugal force increased to 500g. It was determined that the mode of centrifugation at 150 g for 10 minutes using tubes with sodium citrate is the most optimal for obtaining a plasma sample rich in Pl from whole blood and does not cause premature activation of Pl. Based on the obtained data, an algorithm for obtaining PRP from the blood of hemophiliacs and its use for the treatment of patients with HA was developed. Medical and social effect. Medicine. Product Description popup.authors Lanovenko Ivan I. Semeniaka Volodymyr I. popup.nrat_date 2025-01-27 Close