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Registration Number

0225U001571, (0120U100937) , R & D reports

Title

Bioinformatic and molecular-cell studies of the structure and functions of the cytoskeleton of plants

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Біоінформатичні та молекулярно-клітинні дослідження структури та функцій цитоскелету рослин

Head

Blium Yaroslav B., Доктор біологічних наук

Registration Date

03-02-2025

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

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The establishment of molecular-cellular features of the organization and functioning of the plant cytoskeleton, which is the basis of the most significant events of cell ontogenesis, through the integrated use of modern methods of genomics, structural biology and bioinformatics, as well as the introduction of acquired knowledge to create new innovative nano- and biotechnologies, the determination of promising molecular targets and search for appropriate biologically active compounds.

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 Using the integrated use of modern methods of genomics, structural biology and bioinformatics, the molecular and cellular features of the organization and functioning of the plant cytoskeleton were studied, and the theoretical foundation for the creation of new innovative products in nano- and biotechnology was laid. It was shown that the interaction of α-tubulin of representatives of the genus Plasmodium with derivatives of the dinitroaniline series occurs in a site homologous to the binding site of pironetin, and the mechanisms of this interaction are universal for all known isotypes of α-tubulin described for representatives of the genus Plasmodium. The structural and functional variability of the interdomain cleft (IDC) pocket of the FtsZ protein was studied and 89 allosteric inhibitors of the FtsZ protein were identified that directly interact with this site. The direct effect of point polyglutamination on the formation of the interface of interprotein interactions was shown, which in the experiment leads to a decrease in the efficiency of the transport function of microtubules. The acetylome of Arabidopsis thaliana was analyzed and the lysine residues on the surface of the α-tubulin molecule that are subject to acetylation and are most important for stabilizing the microtubule structure were determined using computational methods. A number of plant proteins from the ATG family involved in the formation of the Atg1 complex were modeled and studied, including a thorough analysis of the structure of ATG8 and the prerequisites for the selectivity of its isotypes. It was shown that the kinesins KIN70 (F4J2K4) and KIN1 (Q8GW44) of Arabidopsis are likely transport proteins involved in the autophagy process of higher plants. The evolution of genes for microtubule proteins and proteins associated with the synthesis of cellulose and hemicellulose in higher plants was analyzed by evaluating their untranslated sequences.

Product Description

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Yemets Alla І.
Baier Oleh O.
Borova Mariia M.
Buziashvili Anastasiia Yu.
Bui Denys D.
Horiunova Inna I.
Danylenko Ihor A.
Demchuk Oleh M.
Dmytruk Kostiantyn V.
Karpov Pavlo A.
Kvasko Anna Yu.
Kyrylenko Viktor M.
Kravets Olena A.
Krаsylenko Yuliia A.
Kurylenko Svitlana V.
Kustovskyi Yevhen O.
Melnichuk Oleksandr V.
Novozhylov Dmytro O.
Ozheredov Serhii P.
Pirko Nadiia M.
Pirko Yaroslav V.
Pastuchova Nataliia L.
Pydiura Mykola O.
Plokhovska Svitlana H.
Poiedynok Nataliiia L.
Pushkarova Nadiia O.
Raievskyi Oleksii V.
Samofalova Daria O.
Spivak Svitlana I.
Khablak Serhii H.
Chuhunkova Tetiana V.
Shadrina Ruslana Yu.
Shysha Olena M.

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2025-02-03