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Information × Registration Number 0225U002197, (0124U000078) , R & D reports Title Stress-induced factors of the negative microenvironment as risk drivers of breast cancer progression (code: 2.2.5.460) popup.stage_title Провести біоінформатичний аналіз публічних баз даних та систематизувати відомості щодо профілю експресії генів матриксинів, асоційованого із розвитком та прогресією РМЗ. Дослідити в системі in vitro у клітинних лініях РМЗ людини різного ступеня злоякісності показники експресії генів матриксинів. Head Luk'ianova Nataliia Yu., Доктор біологічних наукChekhun Vasyl F., Доктор медичних наук Registration Date 24-02-2025 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description1 To identify the expression pattern of stress-induced factors of the tumor microenvironment and to substantiate the feasibility of their use to assess the risk of breast cancer progression. popup.description2  In the in silico system, based on the systematization and bioinformatics analysis of public databases (UALCAN, Kaplan-Meier plotter, miRNET v.2.0, mirPath v.3), the expression pattern of matrixin genes, their tissue inhibitors and miRNAs involved in their regulation, associated with the development and progression of breast cancer, was identified. When studying the features of the matrixins and their tissue inhibitors expression in the in vitro system, it was established that the cells of high-malignancy-grade breast cancer lines (MDA-MB-231 and MDA-MB-468) are characterized by a significant increase in the mRNA expression level of matrixins and TIMP2 genes in comparison with similar indicators in cells of low degree of malignancy lines (T47D and MCF-7). In MDA-MB-231 and MDA-MB-468 cell lines, an increase in the expression of gelatinases (MMP-2, MMP-9) against the background of a decrease in the expression of collagenases (MMP-1, MMP-8, and MMP-13) was also recorded on protein levels compared to T47D and MCF-7 cell lines. Based on the results of the in silico bioinformatics analysis, a panel of 6 matrixins associated with the development and progression of breast cancer (MMP-1, MMP-2, MMP-8, MMP-9, MMP-13 and MMP-14) was identified. The results of in vitro studies show that the formation of the malignancy degree in human breast cancer cells is accompanied by a multidirectional change in the expression ratio of collagenases and gelatinases at the mRNA and protein levels, which indicates the feasibility of their further study ex vivo on clinical material as potential markers of the malignant process aggressiveness course. Product Description popup.authors Bazas Volodymyr M. Bozhko Iryna V. Borikun Tetiana V. Burda Tetiana S. Voronina Olena K Hrybach Serhii M. Zadvornyi Taras V. Koziaruk Anastasiia A. Kunska Liubov M. Mushii Oleksandr M. Nalieskina Lesia A. Pavlova Anna O. Panchul Nataliia M. Solomakha Anastasiia I. Ternovyi Mykola K. Trembach Liudmyla V. Shaban Anastasiia A. Shevchuk Anastasiia O. Shepelenko Iryna V. popup.nrat_date 2025-02-24 Close