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Information × Registration Number 0225U002427, (0122U002115) , R & D reports Title Mechanisms of stress adaptation and creation of resistance plant lines by genetic engineering methods popup.stage_title Створення генетичних векторів, що містять гени транскрипційних факторів, що підвищують регенераційну здатність рослин; оптимізація генетичних векторів та отримання трансгенних рослин з генами, експресія яких покращує адаптивні характеристики рослин (тауматин ІІ, рибонуклеаза цинії, ацил-ліпідних десатураз ціанобактерій та ін.) Head Kuchuk Mykola V., д.б.н. Registration Date 11-03-2025 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The aim of the project is to create plants with better adaptive characteristics through genetic transformation of vectors containing genes, the expression of which can increase the resistance of plants to stress. popup.description2  At the first stage of the project implementation, a bioinformatics analysis of a series of genetic sequences was conducted, and vectors for genetic transformation and genome editing of plants using the CRISPR/Cas9 system were created. These vectors aim to enhance plant resistance to various stress factors, specifically phytopathogens, insects, and low temperatures. Additionally, vectors containing maize transcription factor genes (BBM and WUS), which induce somatic embryogenesis, were developed. During the project, the identification and characterization of C-repeat binding factor (CBF) genes in duckweed were carried out. C-repeat binding factors (CBF) play a central role in plant responses to cold, facilitating plant adaptation to low temperatures and increasing freezing tolerance. It was discovered that the expression of the LgCBF1 gene is induced by high osmotic stress and low temperatures, while the expression of LgCBF2 and LgCBF3 fluctuates throughout the day, indicating a circadian rhythm. Furthermore, a study was conducted on the toxicity of the heavy metal manganese (Mn) on Spirodela polyrhiza duckweed, depending on the nitrogen source in the nutrient medium (nitrate or ammonium). The work is based on both well-established approaches (such as the use of cry protein genes toxic to insect pests) and new strategies for creating transgenic plants resistant to stress factors. The vectors developed for plant genetic transformation and genome editing have no analogs in Ukraine. At the first stage of the project implementation, a bioinformatics analysis of a series of genetic sequences was conducted, and vectors for genetic transformation and genome editing of plants using the CRISPR/Cas9 system were created. These vectors aim to enhance plant resistance to various stress factors, specifically phytopathogens, insects, and low temperatures. Additionally, vectors containing maize transcription factor genes (BBM and WUS), which induce somatic embryogenesis, Product Description popup.authors Kishchenko Olena М. Kyrpa Tetiana M. Lystvan Kateryna V. Nifantova Svitlana M. Ovcharenko Olha O. Peterson Anton A. Sindarovska Yana R. Symonenko Yurii V. Shcherbаk Nаtаliia L. popup.nrat_date 2025-03-11 Close