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Information × Registration Number 0226U001547, (0125U002944) , R & D reports Title Genomic evolution of the tubulin gene family in different subspecies of Brassica rapa popup.stage_title Геномна ідентифікація родини генів тубулінів у різних підвидів суріпиці (Brassica rapa) Head Blium Rostyslav Ya., Registration Date 27-01-2026 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description1 The aim of the proposed research work is to study the genomic evolution of the tubulin gene family in different subspecies of Brassica rapa by conducting a comprehensive bioinformatic analysis and molecular genetic analysis. The work involves the identification, structural annotation and comparison of tubulin gene sequences in representatives of different subspecies of Brassica rapa, involving advanced comparative genomics approaches and a large amount of genomic data (up to 30 genome assemblies of B. rapa subspecies). A separate task is to assess the variability of β-tubulin gene introns to identify reliable markers of intron length polymorphism (ILP), which will allow creating an informative system of molecular markers for use in breeding work. In a broader context, the project will allow tracking the evolution of the tubulin gene family within the B. rapa species and gaining new knowledge about the functional role of these genes in the formation of inter-subspecific genetic differentiation. The results obtained will have practical value for plant developmental biology, genomics, systematics, and breeding of cruciferous crops. popup.description2 During the first phase of the study, a genome-wide identification of tubulin gene family members was conducted across various subspecies of Brassica rapa (based on eight available genome assemblies). The analysis included investigation of gene structural organization (exon-intron architecture, chromosomal localization), as well as the establishment of their orthologous relationships and evolutionary links, supported by a series of phylogenetic analyses and whole-genome comparisons. It was also revealed that the genes of certain tubulin isotypes exhibit a high level of intron variation, partly due to the presence of microsatellite sequences. Based on the obtained information regarding intron sequence variability in β-tubulin genes, we assessed the length polymorphism of the first (TBP) and second (cTBP) introns of β-tubulin. The combined TBP/cTBP analysis enabled us not only to differentiate various taxonomic groups of B. rapa cultivars, but also to trace the origin of certain breeding forms of tyfon hybrid. Product Description popup.authors Rostyslav Y. Blume Vladyslava H. Sakharova popup.nrat_date 2026-01-27 Close