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Information × Registration Number 0226U001929, (0124U003709) , R & D reports Title To investigate the molecular-genetic and immunophenotypic characteristics of hematopoietic stem cells used in the process of autologous transplantation in patients with multiple myeloma. popup.stage_title Впровадити дослідження імунофенотипових та молекулярно-генетичних характеристик пухлинного субстрату в кістковому мозку пацієнтів з ММ, яким планується проведення ВДХТ з авто-ТГСК. Розробити методику виявлення залишкових патологічних клонів у концентраті стовбурових гемопоетичних клітин, що застосовується в процесі авто-ТГСК. Head Myhal Ivan I., Доктор філософії Registration Date 03-02-2026 Organization State institution "Institute of blood pathology and transfusion medicine of Nataional Academy of Medical scinces of Ukraine" popup.description1 "To investigate the immunophenotypic and molecular-genetic characteristics of separated hematopoietic stem cells used for hematopoiesis reconstitution after high-dose chemotherapy in patients with multiple myeloma. Establish the relationship between the genotype and immunophenotype of the tumor substrate, the degree of its elimination through different chemotherapy regimes, and the effectiveness of high-dose chemotherapy with autologous stem cell transplantation." popup.description2 In the reporting year 2025 (01 Jan–31 Dec 2025), the activities of the first year of the project were carried out according to the schedule. A clinical cohort of patients with multiple myeloma was formed and expanded; all participants signed informed consent. As of 31 Dec 2025, 30 patients had been enrolled, from which samples were taken. During the year a biobank of biological samples was created, bone marrow specimens were collected and concentrates of hematopoietic stem cells (HSCs) were obtained by apheresis; repeatcollections were treated as new samples without increasing the number of participants. Clinical data were collected and summarized, providing a basis for further correlation analyses between laboratory findings and clinical characteristics. In 2025, molecular genetic investigations were at the preparation stage: organizational activities and reagent procurement were carried out, including the purchasing procedure for next generation sequencing (NGS) reagent kits. The main limiting factor was the lead time for reagent delivery within tender procedures, which delayed the start of NGS analyses but did not hinder other planned work or sample accumulation. Scientific outputs were reflected in publication activity: 2 conference abstracts were prepared and published and 1 research article was published. Thus, in 2025 the key tasks of the stage were accomplished with regard to cohort formation, biobanking and immunophenotyping, and an organizational and material basis was established for the subsequent NGS stage and comprehensive analysis once reagents are received. Interim results support the feasibility of the chosen approach and ensure continuity of the project in the next period. The accumulated sample set and harmonized clinical dataset will be used to interpret future NGS results and to refine the study workflow as the project progresses Product Description popup.authors Thir Hrystyna R. Khudzii Sofiia S. Halyna R. Savuliak Shmyhelska Solomiia M. popup.nrat_date 2026-02-03 Close