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Information × Registration Number 0226U002611, (0125U000646) , R & D reports Title Optimization of conditions of interaction of biological objects with nanostructured materials as a basis for studying their biosafety popup.stage_title Провести ґрунтовний аналіз норм затверджених міжнародних документів, що регламентують застосування наноструктурних матеріалів, в аспекті оцінки їх безпечності. Обґрунтувати методологічний підхід до оцінки ризиків взаємодії біооб’єктів з наноматеріалами. Вивчити вплив матеріалів на загальні показники токсичності in vitro та in vivo Head Zahorodnia Svitlana D., Кандидат біологічних наук Registration Date 02-03-2026 Organization Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine popup.description1 Identification of the peculiarities of the interaction between biological objects and nanostructured materials, as well as the development of methodological recommendations for interpreting the results of studying their biosafety based on practically developed methodological approaches. popup.description2 Research report: 37 pages, 5 tables, 5 figures, 51 sources. CELLS, CYTOTOXICITY, NANOPARTICLES, NANOSTRUCTURES Research objects – silver nanoparticles with a size of 10 nm, cultures of monolayer transplantable cells: bovine kidney (MDBK), dog kidney (MDCK) and Syrian hamster kidney (VNK-21), Balb/C/ mice The aim of the work is to analyze the norms of approved international documents regulating the use of nanostructured materials in terms of assessing their safety. Based on the literature search, to justify a methodological approach to assessing the risks of interaction between bio-objects and nanomaterials. To study the effect of materials on general toxicity indicators in vitro and in vivo Research methods: bibliosemantic, cytological, molecular biological, hematological, biochemical, statistical. An analysis of regulatory and legal regulation in the field of nanotechnology and scientific publications devoted to testing and standardization of nanoparticles and nanomaterials was carried out using electronic resources and databases of WHO, PubMed using the bibliosemantic method. The study examined the cytotoxic effects of 10 nm silver nanoparticles using neutral red and 3-(4-5 dimethyltriazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dyes. The dependence of the cytotoxic effect of nanoparticles on stabilizing solutions was demonstrated. In vivo toxicological studies were conducted. It was shown that nanosilver is toxic when administered intraperitoneally in high doses. When administered orally and intravenously, it does not cause death in animals at the maximum doses studied. In all administration options, changes in biochemical and hematological parameters were observed, which were dose-dependent and dynamic over time. Product Description popup.authors Krystyna S. Naumenko Tetiana V. Zatovska Halyna V. Baranova Polina Y. Zaremba Andrii A. Zaremba Liubov O. Artiukh Olha Y. Povnytsia Nina Y. Tkachenko Anhelina P. Nyzhenets Oleksandra V. Lychak Alona Y. Bokovnia Liubov B. Zelena Valentyna I. Savon Liudmyla M. Podorozhnia Hanna S. Halan popup.nrat_date 2026-03-02 Close