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Information × Registration Number 0304U001116, 0103U008711 , R & D reports Title Heterochromatin structural organization, its variability and influence on the genome dedifferentiation and reorganization processes in plant tissue culture in vitro popup.stage_title Вивчення особливостей структурної організації конститутивного гетерохроматину, різного за функціями (теломерного, субтеломерного). Аналіз здатності до дефиренціювання та калюсоутворення in vitro клітин рослин, що відрізняються за кількістю та розподілом конститутивного гетерохроматину в метафазних хромосомах та інтерфазних ядрах. Дослідження особливостей реорганізації геному в процесах дедиференціювання та тривалого вирощування клітин in vitro. Head Kunakh Viktor Anatoliyovich, Registration Date 22-01-2004 Organization Institute of molecular biology & genetics popup.description2 Object - plant nuclear DNA. Goal - investigation of higher order organization of heterochromatic regions of plant genome. Methods - isolation of intact nuclear DNA, pulsed field gel electrophoresis, blot-hybridization. To study the higher order chromatin organization of rye DNA a principally new approach was applied. The approach is based on the fractionation in pulsed field gel electrophoresis of DNA cleaved by the endogenous nucleases in NaCl-extracted nuclei. An investigation of structural organization of rye genome regions, which consist of high-repeated sequences, showed that the regions is organized in loop domains of regular size by DNA attachment to the nuclear matrix. The data obtained suggest that genome regions that consist of different repeats form DNA loops differed in size. The lengths of high-molecular weight DNA fragments, which was formed by the cleavage with endogenous nucleases of genome regions consisted of high repeats, were stable in physiologically different tissues. This resultsuggests the significant stability of domain organization of heterochromatic chromosome regions. Transition of plant cells from proliferatively active state to differentiation accompanied with increase of both high molecular DNA cleavage and heterochromatic chromosome regions by endogenous nucleases. HETEROCHROMATIN, STRUCTURAL ORGANIZATION OF CHROMATIN, REPEATED DNA SEQUENCES, PULSED FIELD GEL ELECTROPHORESIS, DNA-TOPOISOMERASE II, SECALE CEREALE. Product Description popup.authors popup.nrat_date 2020-04-02 Close