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Information × Registration Number 0313U005073, 0111U001819 , R & D reports Title Use of effective oligoelectrolyte systems for delivery of genes into cells for genetic transformation of plants popup.stage_title Адаптація розроблених систем для доставки нуклеїнових кислот в клітини суспензійної культури рослин, калуса, експлантів рослин, дослідження властивостей комплексу ДНК-полімерний носій Head Yemets A. I., Registration Date 11-06-2013 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 It was adapted protocols for foreign DNA transfer using poly-DMAEM nanocarriers considering the presence of the cell wall as an additional barrier used in recipients. It aws studied the effect of different concentrations of polymer carrier poly-DMAEM type BG-2 on cell suspension culture and callus lines of tobacco (Nicotiana tabacum) BY-2. It was established that the polymer carriers of poly-DMAEM type BG-2 have a negative impact in high concentrations on the cell membrane suspension culture and callus tissue and affect their viability. We recommend using the concentration of polymer carriers such as BG-2 in the range of 10-100 mg for further transformation of this cell type. The possibility of using the poly-DMAEM for genetic transformation of protoplasts of the moss Physcomitrella patens and Ceratodon purpureus was confirmed. Product Description popup.authors Ємець А. І. Блюм Я.Б. Танасієнко І.В. popup.nrat_date 2020-04-02 Close