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Information × Registration Number 0314U004141, 0114U005237 , R & D reports Title Investigation of the direct and indirect action of the radiation exposureon the biological systems popup.stage_title Вивчення властивостей модельних ліпосомних систем фосфатидилхоліну з насіння соняшника Head Shulga Sergiy Michaylovich, Кандидат фізико-математичних наук Registration Date 08-07-2014 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 Aim of the project: determination of quality and quantitative composition of components of deoil sunflower lecithin, extraction of pure phosphatidylcholine (98%), creation of "empty" stable liposome. Terms of division of phosphotides with detection in UV range certain by methods HPLC. Possibility of application the reversephase and normalphase modes was investigated by HPLC. The phosphotides exit times during the analysis of standard mixture are determind and the phosphotides of deoil sunflower lecithin are identified at times retaining of corresponding components of standard mixture. Methodology of determination of PC, PE, PI by HPLC at the normalphase mode was investigated and quantitative content of PC, PE, PI in the lecithin of sunflower was determined. The components of lecithin of sunflower are identified by means of tandem mass-spectrometry method and fat acids components composition was certain. The method of homogenization (extrusions) at corresponding terms is get "empty" liposomes. The dimension of obtained liposomes by the methods of atomic force microscopy and laser photon-correlation spectroscopy were determined. Liposomes from sunflower phosphatidylcholine are stable at storage and on the process of oxidization was shown Product Description popup.authors Іващенко Т. Андріяш Г. Бейко Н. Блюм Я.Б. Заболотна Г.М. Круподьорова Т. Прийомов С.І Сорочинський Б. Тігунова О. Ткаченко А. Хоменко А. Шульга С.М. popup.nrat_date 2020-04-02 Close