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0402U003027,
к.б.н.
15-10-2002
o
The investigation of functional properties of the latrotoxin-like protein as a possible participator of the process of neurosecretion
Glyvuk Nataliya Valentinovna,
popup.head Malysheva Margarita Konstantinovna
popup.opponent Кришталь Олег Олександрович
popup.opponent Громов Леонід Олександрович
popup.opponent Веселовський Микола Сергійович
. В роботі показано, що латротоксинподібний білок збільшує ступінь АТФ - викликаного злиття гомотипових мембран. Ця властивість латротоксинподібного білка може модулюватися процесами фосфорилювання-дефосфорилювання, зміною рН середовища та шляхом обробки білка N-этилмаліємідом. Встановлено, що латротоксинподібному білку притаманна АТФазна активність, характерна для білків ААА родини АТФаз. Показано, що аплікація латротоксинподібного білка призводила до значного збільшення амплітуди кальцієвого струму Р-типу та дещо меньшому зростанню струму L-типу. Дослідження впливу на спонтанні постсинаптичні відповіді показали, що аплікація білка призводила до деякого зростання амплітуди та значного збільшення частоти GABAергічних IPSC. Таким чином латротоксинподібний білок можливо здатен брати участь в процесі синаптичної передачі.
2002-10-15
2020-04-04
Updated: 2025-12-07
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