Текст. Pantyo V. V.. Impact of methylene blue and LED radiation on microbial biofilms

Реєстраційний номер

2125U009243, Матеріали видань та локальних репозитаріїв

Категорія

Текст

Назва роботи

Impact of methylene blue and LED radiation on microbial biofilms

Автор

Pantyo V. V.
Пантьо Валерій Валерійович
Pallah O. V.
Паллаг Олександра Володимирівна
Boiko N. V.
Бойко Надія Володимирівна
Danko E. M.
Данко Ельвіра Михайлівна
Koval G. M.
Коваль Галина Миколаївна
Pantyo V. I.
Пантьо Валерій Іванович
Chobey A. S.
Чобей Андрій Степанович
Pantyo V. V.
Panto Valerii Valeriiovych
Pallah O. V.
Pallah Oleksandra Volodymyrivna
Boiko N. V.
Boiko Nadiya Volodymyrivna
Danko E. M.
Danko Elvira Mykhailivna
Koval G. M.
Koval Halyna Mykolaivna
Pantyo V. I.
Panto Valerii Ivanovych
Chobey A. S.
Chobei Andrii Stepanovych

Дата публікації

01-01-2025

Постачальник інформації

Ужгородський національний університет

Першоджерело

https://dspace.uzhnu.edu.ua/handle/lib/79031

Видання

Oles Honchar Dnipro National University

Опис

Antibiotics play a key role in treating and controlling infectious diseases caused by pathogenic bacteria. Overconsumption and misuse of antibiotics have led to an increase in bacterial drug resistance. Resistant microorganisms are responsible for infec-tions that are more difficult and expensive to treat; besides multidrug-resistant microorganisms cause hundreds of thousands of deaths annually. To combat antibiotic resistance, physicochemical methods, including antimicrobial photodynamic therapy, are becoming increasingly widespread. The purpose of the study was to determine the complex effect of methylene blue as a photo-sensitizer, and LED radiation on the biofilms of some opportunistic microorganisms. To examine biofilm formation and the impact of photodynamic therapy on formed biofilms, the microtiter plate method was used. The obtained results showed that 20-minute exposure of LED light of red-infrared spectrum did not cause statistically significant effect on formed biofilms. Photosen-sitizer methylene blue alone caused a certain antibiofilm effect which was expressed in a decrease in the growth intensity of bio-film scrapings after inoculation of appropriate dilutions into the Petri dishes on average by 33.2–60.0%, compared to the control group. The combined action of the photosensitizer with subsequent irradiation of biofilms by LED radiation demonstrated pro-nounced antibiofilm activity, which was much higher than the separate effects of specified factors. In this case, the growth inten-sity of the studied microorganisms at similar dilutions of biofilm scrapings decreased on average by 65.8–92.5%. Investigated strains of Staphylococcus aureus and Candida albicans were more susceptible than Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa. Due to the lack of pronounced side effects and the presence of several advantages, including wide-spectrum antimicrobial and antibiofilm activity, antimicrobial photodynamic therapy can be used in the treatment of inflammato-ry-purulent processes particularly in dentistry and dermatology.

Додано/оновлено в НРАТ

2026-06-13