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Information × Registration Number 0124U000667, ( 0226U001533  ) R & D request Title Development of a test kit for the detection of the genetic material of the causative agent of anthrax based on quantitative PCR and recombinant technologies with the aim of improving the disease control system in conditions of armed aggression by the Russian Federation and post-war recovery of Ukraine Head Biloivan Oleksandr V., Кандидат ветеринарних наук Registration Date 22-01-2024 Organization National Scientific Center Institute of Experimental and Clinical Medicine popup.description1 The goal of the project is to create a test kit for detecting the DNA of the anthrax pathogen in biological material and environmental samples using the PCR method. The test kit is planned to be based on recombinant positive control samples of specific DNA fragments of the anthrax pathogen, namely the chromosomal marker dhp61, the pagA gene fragment of the pXO1 plasmid, and the capC gene fragment of the pXO2 plasmid of Bacillus anthracis. The amplification parameters will be optimized, as well as the concentrations of reaction mixture components. Coefficients of variation within one and several independent experiments will be determined. Analytical, diagnostic sensitivity, and specificity of the method will be established. popup.nrat_date 2024-12-09 Close